Part:BBa_K103020:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

'Hunter and prey' selection system

This part was used as core of prey generator device. It was fused with protein Z_SPA-1 BBa_K103004. Resulting fusion was named His_omega_Z

The fusion was overexpressed in Rosetta strain. Due to ability of protein Z to form aggregates His_omega_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. The fusion was added to culture media and used in selection experiments. Details here and here.

User Reviews

UNIQ45fde1aef35d8e5f-partinfo-00000000-QINU UNIQ45fde1aef35d8e5f-partinfo-00000001-QINU